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(1) Column Chromatography Column Chromatography is another general and effective separation techni'ue in organicchemistry. This separation process involves the identical ethics as T"C, but can be applied toseparate larger amounts than T"C. Column chromatography can be used on both a large andsmall range. The appeal of this techni'ue are ample prolong and across many systems, including biology, biochemistry, microbiology and medicine. any general antibiotics are reformed bycolumn chromatography. To understand to use of this separation techni'ue, we can conduct thelast test as an e3ample. 4n the T"C test, we separated and analyzed the various elements thatma5e up over6the6counter pain5illers. The techni'ue of T"C was useful in fi3ing the type andnumber of ingredients in the composition, but it was not helpful for collecting the separatedingredients. 7e could only separate and see the stage. 4f we needed to collect the separatedingredients, column chromatography could be used. 7e could load 100 mg of a crushed -nacintablet on a column made up of a silica stationary phase and separates the aspirin from thecaffeine and collect each of these compounds in separate bea5ers. Column chromatographyallows us to separate and collect the compounds individually. 4n this test, ColumnChromatography (abbreviated CC) will be used to separate the starting material from the productin the o3idation of fluorene to flourenone and T"C will be used to monitor the usefulness of thisseparation
(2) Paper Chromatography
This principle connected to the partition chromatography wherein the substances are distributedor partitioned among li'uid phases. 8ne phase is the water, which is held in the pores of the filter paper used9 and the other is the mobile phase which moves over the paper. The compounds in themi3ture get separated due to differences in their affinity towards water (in stationary phase) andmobile phase solvents during the movement of mobile phase under the capillary action of poresin the paper.The ethics can also be adsorption chromatography between solid and li'uid phases, where thestationary phase is the solid surface of paper and the li'uid phase is of mobile phase. :ut most of the appeal of paper chromatography action of the principle of partition chromatography, i.e. partitioned between two li'uid phases.